Column:

Target  Phenyl-Hexyl , 5 μm,  4.6 × 250 mm

Mobile phase:

A: ACN       B: 0.5%CH3COOH

Time(min): 
    B(%):

0     15    30    40  
90    80    80    70

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 350 nm

Injection Volume:

10 μL

Sample:

Flos Lonicerae Japonicae extract 
1. Galuteolin （木犀草苷）